Given that the 16S profiles within the intestine appear very diverse for intracellular and extracellular DNA [33], qPCR is anticipated to replicate the NGS-specific Group framework both equally quantitatively and qualitatively far more carefully than flow cytometry.
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It should be mentioned that relative and absolute abundances based upon 16S rRNA gene copies absolutely are a proxy for microbial density in lieu of correct numeration of cells, given that 16S rRNA gene copy numbers vary between microbes. It is actually, nonetheless, achievable to computationally accurate for 16S rRNA gene copy quantities article hoc
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